rat anti human cd28  (Bio-Rad)

Journal: Cellular and Molecular Gastroenterology and Hepatology

Article Title: Role of ICAM-1 in the Adhesion of T Cells to Enteric Glia: Perspectives in the Formation of Plexitis in Crohn’s Disease

doi: 10.1016/j.jcmgh.2024.02.016

Figure Lengend Snippet: List of Antibodies Used in the Study

Article Snippet: T-cell activation was performed using anti-CD28 (2.5 [rat] or 5 [human] μg/mL) and coated anti-CD3 (1 μg/mL) antibodies (BD-Bioscience).

Techniques: Activation Assay

Journal: Scientific Reports

Article Title: CEACAM1 mediates B cell aggregation in central nervous system autoimmunity

doi: 10.1038/srep29847

Figure Lengend Snippet: ( A ) Gating strategy. ( B–G ) CEACAM1 expression on naïve ( B,E ) and memory B cells ( C,F ) as well as on B1 cells ( D,G ). MS patients were tested during remission ( n = 19) ( B–D ) and relapse ( n = 8) ( E–G ). Statistical significance was determined by Wilcoxon rank sum test. ( H–K ) CEACAM1/TIM-3 coexpression on B cells ( H ), cytotoxic T lymphocytes (CTLs, I ) and T helper cells (T H , J,K ) before ( J ) and after ( K ) anti-CD3/anti-CD28 antibody stimulation. Samples were analyzed from patients during remission that received treatment with natalizumab (before stimulation n = 10, after stimulation n = 8). Healthy controls ( n = 8 , n = 7) were age- and gender-matched. Patients in remission all received natalizumab ( n = 19), while patients in relapse received either natalizumab ( n = 1), interferon-β ( n = 2) or were untreated ( n = 5).

Article Snippet: PBMCs were isolated by density gradient centrifugation and stained immediately and after T cell stimulation with mouse anti-human anti-CD3 (clone CD3-2; Mabtech) and rat anti-human CD28 (clone CD28-A; Mabtech) at 0.1 μg/ml for 72 h at 37 °C and 7% CO 2.

Techniques: Expressing

Journal: Acta dermato-venereologica

Article Title: Systemically elevated Th1-, Th2- and Th17-associated chemokines in psoriasis vulgaris before and after ultraviolet B treatment.

doi: 10.2340/00015555-1545

Figure Lengend Snippet: Fig. 3. Levels of the cytokines interleukin (IL)-10, IL-13, IL-17A and interferon (IFN)-γ in cell culture supernatant from anti-CD3-, anti-CD28-activated peripheral blood mononuclear cells (PBMCs) from patients with psoriasis before (Psoriasis CD3CD28 0 wks) and 12 weeks after narrowband ultraviolet B (UVB) therapy (Psoriasis CD3CD28 12 wks) and from healthy controls (Healthy CD3CD28). The line shows the median, n = 10.

Article Snippet: After washing, a total of 1 × 106 cells were added to each well and cultured for 72 h with 5 μg/ml rat monoclonal anti-human CD28 antibody (isotype IgG2b, AbD Serotec).

Techniques: Cell Culture